Unstained protein ladder western blot4/18/2024 ![]() ![]() The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. Use the same volumes for Western blotting. Load the following volumes of the ladder on an SDS-polyacrylamide gel: 5 L per well for mini gel, 10 L per well for large gel. Supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.Ĭaution: Not intended for human or animal diagnostic or therapeutic uses. Mix gently, but thoroughly, to ensure the solution is homogeneous. The apparent molecular weight of each protein has been determined by calibration against unstained protein standardsĢ. Each protein in the ladder contains an integral Strep-tag. Migration patterns of BLUelf Prestained Protein Ladder in different electrophoresis conditions are listed below:ġ. purified, unstained proteins for use as size standards in SDS-PAGE and western blotting. Guide for Molecular Weight Estimation (kDa) Protein ladders for western blotting and fluorescence western blotting. Apply more for thicker (> 1.5 mm) or larger gel On this page: Prestained broad and high molecular weight protein ladders.10 μl per well for large gels, 5 μl per well for blots.5 μl per well for mini-gels, 2.5 μl per well for blots.Load the following volumes of the ladder on SDS-PAGE gel: Mix thoroughly to ensure the solution is homogeneous.ģ. Thaw the ladder either at room temperature or at 37-40☌ for a few minutes to dissolve precipitated solids. Do not boil.Ģ. The apparent molecular weight of each protein was determined by calibration. The quality of the BLUelf Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality.īLUelf Prestained Protein Ladder Protocolġ. Western Blot View all Secondary Antibodies Proteins & Peptides. Sizing of proteins on SDS-PAGE and Western blots.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.Monitoring of protein transfer onto membranes during Western blots.Monitoring of protein migration during SDS-PAGE. An equal amount (8 l) of supernatant from each sample and control were loaded into each separate well on SDS-PAGE gels, and 4 l of PageRuler Unstained Protein Ladder (Thermo Fisher Scientific, Massachusetts, USA) was also included in each gel.Features of PageRuler Prestained Protein Ladder. Easy to identify: includes the ~25, ~75 kDa reference bands coupled with a green and a red dye proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.Ready-to-use: supplied in a loading buffer for direct loading on gels.Broad range: 3.5-245 kDa (Tris-glycine-SDS running buffer).Do not heats, dilute, and add reducing agent before loading. The ladder is supplied in gel loading buffer and is ready to use. The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of p roteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with Tris-glycine-SDS running buffer. Precision Plus Protein Unstained Standard Plugs, Strep-tagged, recombinant, pkg of 24 ( 1610378)įor blot development, Precision Protein™ StrepTactin-HRP Conjugate or StrepTactin-AP Conjugate should also be ordered.The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 prestained proteins covering a wide range molecular weights from 3.5 to 245 kilodalton (kDa). Protein Molecular Weight Marker wide Range (prestained) is ideal for precise sizing of proteins by SDS-PAGE and for Western blotting, protein marker.Standard plugs are offered for 2-D electrophoresis applications: The unique Strep-tag affinity peptide allows detection and molecular weight determination on western blots. Browse Bio-Rads wide range of quality prestained and unstained protein standards. The unique Strep -tag affinity peptide allows detection and molecular weight determination on western blots. Details on blotting technology, available products, and tips and techniques. Precision Plus Protein Unstained Standards are a mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, and 75 kD). Precision Plus Protein Unstained Standards are a mixture of ten Strep-tagged, recombinant proteins (10250 kD), including three reference bands (25, 50, and 75 kD). ![]() Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. ![]()
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